Antibody FAQs

How much sample should be loaded?

 Cell lysates, membrane and nuclear lysates: load 20 to 30 μg of total protein per well. This may require some optimization depending on the expression level of the protein in the sample you are testing. Purified protein (recombinant or endogenous): load 10 to 100 ng of protein per well. 

Do you recommend blocking with milk or BSA? 

Unless specifically indicated in the data sheet we recommend blocking with 1X TBS/T with 5% w/v nonfat dry milk. Blocking with BSA or Tween 20 has application for phospho-specific antibodies; in this case Milk should not be used. 

How do I know this antibody is specific for Protein X? Does it cross-react with ProteinX1, X2, X3, X4? 

Check the specificity for target using about 50 amino acid immunogen region sequence in a protein BLAST homology search. If you are unsure it, please contact technical support for assistance.